East Coast Fever (ECF), caused by Theileria parva, is a major constraint to improved livestock keeping in east and central Africa, including Zambia. To understand the dynamics and determine the candidates for immunization in Zambia’s Chongwe and Chisamba districts, a combination of Tp1 and Tp2 gene sequencing and microsatellite analysis using nine markers was conducted from which an abundance of Muguga, Kiambu, Serengeti and Katete epitopes in the field samples was obtained. Phylogenetic analysis showed six (Tp1) and three (Tp2) clusters with an absence of geographical origin clustering. The majority of haplotypes were related to Muguga, Kiambu, Serengeti and Katete, and only a few were related to Chitongo. Both antigens showed purifying selection with an absence of positive selection sites. Furthermore, low to moderate genetic differentiation was observed among and within the populations, and when vaccine stocks were compared with field samples, Chongwe samples showed more similarity to Katete and less to Chitongo, while Chisamba samples showed similarity to both Katete and Chitongo and not to Muguga, Kiambu or Serengeti. We conclude that the use of Katete stock for immunization trials in both Chongwe and Chisamba districts might produce desirable protection against ECF.
Sequence Diversity of Tp1 and Tp2 Antigens and Population Genetic Analysis of Theileria parva in Unvaccinated Cattle in Zambia’s Chongwe and Chisamba Districts
One of the core mandates of CTTBD is to conduct tailored courses and training in Animal health, diagnostics and tick-borne disease control. In mid-2019, CTTBD entered into a partnership with
Molecular characterization and population genetics of Theileria parva in Burundi’s unvaccinated cattle: Towards the introduction of East Coast fever vaccineMolecular characterization and population genetics of Theileria parva in Burundi’s unvaccinated cattle: Towards the introduction of East Coast fever vaccine
CTTBD EXPLORES THE USE OF DRY ICE AS AN ALTERNATIVE TO LIQUID NITROGEN FOR STORAGE OF EAST COAST FEVER VACCINESCTTBD EXPLORES THE USE OF DRY ICE AS AN ALTERNATIVE TO LIQUID NITROGEN FOR STORAGE OF EAST COAST FEVER VACCINES
The Centre for Ticks & Tick-borne Diseases (CTTBD), an institution based in Lilongwe, Malawi, has a mandate to support the control of livestock diseases and the development of the livestock